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dapi scientific laboratory d9542  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc dapi scientific laboratory d9542
    Dapi Scientific Laboratory D9542, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+d9542/DAPI/pm41422054-367-10-16
    Average 98 stars, based on 2155 article reviews
    dapi scientific laboratory d9542 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Staining:

    Article Title: Lateral cell polarization drives organization of epithelia in sea anemone embryos and embryonic cell aggregates
    Article Snippet: The following secondary antibodies were all used in 1:1000 concentration: goat anti mouse Alexa Fluor 488 antibody (Invitrogen, A11001), goat anti-mouse Alexa Fluor 568 antibody (Invitrogen, A11004), goat anti-rabbit Alexa Fluor 488 (Invitrogen, A11008) and goat anti-rabbit Alexa Fluor 555 (Invitrogen, A21428). .. DNA was stained with either DAPI (1μg/ml, Sigma D9542) or Draq5 (1:2000, Cell Signaling 4084) and F-actin was stained with Alexa Fluor 488, 555 or 647 phalloidin (1:200, Invitrogen A12379, A34055 or A22287, respectively) that were added in the overnight secondary antibody incubation at 4oC. ..

    Article Title: Lateral cell polarization drives organization of epithelia in sea anemone embryos and embryonic cell aggregates
    Article Snippet: The following secondary antibodies were all used in 1:1000 concentration: goat anti mouse Alexa Fluor 488 antibody (Invitrogen, A11001), goat anti-mouse Alexa Fluor 568 antibody (Invitrogen, A11004), goat anti-rabbit Alexa Fluor 488 (Invitrogen, A11008) and goat anti-rabbit Alexa Fluor 555 (Invitrogen, A21428). .. DNA was stained with either DAPI (1μg/ml, Sigma D9542) or Draq5 (1:2000, Cell Signaling 4084) and F-actin was stained with Alexa Fluor 488, 555 or 647 phalloidin (1:200, Invitrogen A12379, A34055 or A22287, respectively) that were added in the overnight secondary antibody incubation at 4°C. ..

    Incubation:

    Article Title: Lateral cell polarization drives organization of epithelia in sea anemone embryos and embryonic cell aggregates
    Article Snippet: The following secondary antibodies were all used in 1:1000 concentration: goat anti mouse Alexa Fluor 488 antibody (Invitrogen, A11001), goat anti-mouse Alexa Fluor 568 antibody (Invitrogen, A11004), goat anti-rabbit Alexa Fluor 488 (Invitrogen, A11008) and goat anti-rabbit Alexa Fluor 555 (Invitrogen, A21428). .. DNA was stained with either DAPI (1μg/ml, Sigma D9542) or Draq5 (1:2000, Cell Signaling 4084) and F-actin was stained with Alexa Fluor 488, 555 or 647 phalloidin (1:200, Invitrogen A12379, A34055 or A22287, respectively) that were added in the overnight secondary antibody incubation at 4oC. ..

    Article Title: Lateral cell polarization drives organization of epithelia in sea anemone embryos and embryonic cell aggregates
    Article Snippet: The following secondary antibodies were all used in 1:1000 concentration: goat anti mouse Alexa Fluor 488 antibody (Invitrogen, A11001), goat anti-mouse Alexa Fluor 568 antibody (Invitrogen, A11004), goat anti-rabbit Alexa Fluor 488 (Invitrogen, A11008) and goat anti-rabbit Alexa Fluor 555 (Invitrogen, A21428). .. DNA was stained with either DAPI (1μg/ml, Sigma D9542) or Draq5 (1:2000, Cell Signaling 4084) and F-actin was stained with Alexa Fluor 488, 555 or 647 phalloidin (1:200, Invitrogen A12379, A34055 or A22287, respectively) that were added in the overnight secondary antibody incubation at 4°C. ..

    MTT Assay:

    Article Title: Flubendazole Enhances the Inhibitory Effect of Paclitaxel via HIF1α/PI3K/AKT Signaling Pathways in Breast Cancer.
    Article Snippet: MCF-7 and MDA-MB-231 originated from ATCC and were maintained in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (Life Technologies, Carlsbad, CA, USA) at 37 ◦C in 5% CO2. .. Reagents used in this study were as follows: MTT (M2128), DAPI (D9542), Flubendazole (HY-B0294), Paclitaxel (HY-B0015), MMP-2 (87809, CST), E-cadherin (14472, CST), β-actin (66009-1-Ig, Proteintech, Rosemont, IL, USA), N-Cadherin (13116, CST), PI3K (4255, CST), p-PI3K (17366, CST), AKT (9272, CST), p-AKT (4060S, CST), mTOR (2972, CST), p-mTOR (5536, CST), HIF1α (36169, CST) p62 (88588, CST), LC3 (4108, CST), ATG5 (12994, CST). ..

    Article Title: Flubendazole Enhances the Inhibitory Effect of Paclitaxel via HIF1α/PI3K/AKT Signaling Pathways in Breast Cancer
    Article Snippet: MCF-7 and MDA-MB-231 originated from ATCC and were maintained in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (Life Technologies, Carlsbad, CA, USA) at 37 °C in 5% CO 2 . .. Reagents used in this study were as follows: MTT (M2128), DAPI (D9542), Flubendazole (HY-B0294), Paclitaxel (HY-B0015), MMP-2 (87809, CST), E-cadherin (14472, CST), β-actin (66009-1-Ig, Proteintech, Rosemont, IL, USA), N-Cadherin (13116, CST), PI3K (4255, CST), p-PI3K (17366, CST), AKT (9272, CST), p-AKT (4060S, CST), mTOR (2972, CST), p-mTOR (5536, CST), HIF1α (36169, CST) p62 (88588, CST), LC3 (4108, CST), ATG5 (12994, CST). ..

    Cell-Signaling:

    Article Title: A High Immune-Related Index with the Suppression of cGAS-STING Pathway is a Key Determinant to Herceptin Resistance in HER2+ Breast Cancer
    Article Snippet: .. Table S1: Key Resources Antibodies Source Identifier Dilution IRF3 Proteintech 11312-1-AP-100UL 1:1000 HER2 Proteintech 18299-1-AP-50ul 1:1000 Phospho-IRF3 (Ser396) Abcam Ab138449 1:1000 cGAS Proteintech 26416-1-AP-100ul 1:1000 TBK1 Cell signaling technology 38066S 1:1000 AKT1 (B-1) Santa Cruz sc-5298 1:500 Phospho-TBK1 (Ser172) Cell signaling technology 5483S 1:1000 Phospho-AKT (Ser473) Cell signaling technology 4060S 1:1000 GAPDH Proteintech 60004-1-IG-100UL 1:5000 α-tubulin Beyotime AT819 1:5000 Anti-human HLA-A, B, C BioLegend 311406 1:300 CD3 BioLegend 300313 1:500 CD28 BioLegend 302913 1:500 CD8a Proteintech 66868-1-IG 1:300 GZMB Proteintech 13588-1-AP 1:300 Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Invitrogen 11668019 HT-DNA Sigma-Aldrich D6898 2’3’-cGAMP Titan 04800926 Poly(I:C)/dsRNA InvivoGen tlrl-picwlv Poly(dA:dT) / LyoVec InvivoGen tlrl-patc Human IL-2 PeproTech 200-02-100 RIPA buffer Cell signaling technology 9806s DAPI Sigma-Aldrich D9542 ADU-S100 MCE HY-12885B Critical commercial assays 2’3’-cGAMP ELISA Kit Cayman 501700 RNA Purification Kit EZBioscience HiScript II QRT SuperMix Vazyme R223-01 ChamQ-SYBR-qPCR-MasterMix Vazyme Q311-02 qPCR Primers (human) Forward (5’>3’) Reverse (5’>3’) ..

    Recombinant:

    Article Title: A High Immune-Related Index with the Suppression of cGAS-STING Pathway is a Key Determinant to Herceptin Resistance in HER2+ Breast Cancer
    Article Snippet: .. Table S1: Key Resources Antibodies Source Identifier Dilution IRF3 Proteintech 11312-1-AP-100UL 1:1000 HER2 Proteintech 18299-1-AP-50ul 1:1000 Phospho-IRF3 (Ser396) Abcam Ab138449 1:1000 cGAS Proteintech 26416-1-AP-100ul 1:1000 TBK1 Cell signaling technology 38066S 1:1000 AKT1 (B-1) Santa Cruz sc-5298 1:500 Phospho-TBK1 (Ser172) Cell signaling technology 5483S 1:1000 Phospho-AKT (Ser473) Cell signaling technology 4060S 1:1000 GAPDH Proteintech 60004-1-IG-100UL 1:5000 α-tubulin Beyotime AT819 1:5000 Anti-human HLA-A, B, C BioLegend 311406 1:300 CD3 BioLegend 300313 1:500 CD28 BioLegend 302913 1:500 CD8a Proteintech 66868-1-IG 1:300 GZMB Proteintech 13588-1-AP 1:300 Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Invitrogen 11668019 HT-DNA Sigma-Aldrich D6898 2’3’-cGAMP Titan 04800926 Poly(I:C)/dsRNA InvivoGen tlrl-picwlv Poly(dA:dT) / LyoVec InvivoGen tlrl-patc Human IL-2 PeproTech 200-02-100 RIPA buffer Cell signaling technology 9806s DAPI Sigma-Aldrich D9542 ADU-S100 MCE HY-12885B Critical commercial assays 2’3’-cGAMP ELISA Kit Cayman 501700 RNA Purification Kit EZBioscience HiScript II QRT SuperMix Vazyme R223-01 ChamQ-SYBR-qPCR-MasterMix Vazyme Q311-02 qPCR Primers (human) Forward (5’>3’) Reverse (5’>3’) ..

    Article Title: Brain-wide mapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL-17RB.
    Article Snippet: Article Brain-widemapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL17RB

    Enzyme-linked Immunosorbent Assay:

    Article Title: A High Immune-Related Index with the Suppression of cGAS-STING Pathway is a Key Determinant to Herceptin Resistance in HER2+ Breast Cancer
    Article Snippet: .. Table S1: Key Resources Antibodies Source Identifier Dilution IRF3 Proteintech 11312-1-AP-100UL 1:1000 HER2 Proteintech 18299-1-AP-50ul 1:1000 Phospho-IRF3 (Ser396) Abcam Ab138449 1:1000 cGAS Proteintech 26416-1-AP-100ul 1:1000 TBK1 Cell signaling technology 38066S 1:1000 AKT1 (B-1) Santa Cruz sc-5298 1:500 Phospho-TBK1 (Ser172) Cell signaling technology 5483S 1:1000 Phospho-AKT (Ser473) Cell signaling technology 4060S 1:1000 GAPDH Proteintech 60004-1-IG-100UL 1:5000 α-tubulin Beyotime AT819 1:5000 Anti-human HLA-A, B, C BioLegend 311406 1:300 CD3 BioLegend 300313 1:500 CD28 BioLegend 302913 1:500 CD8a Proteintech 66868-1-IG 1:300 GZMB Proteintech 13588-1-AP 1:300 Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Invitrogen 11668019 HT-DNA Sigma-Aldrich D6898 2’3’-cGAMP Titan 04800926 Poly(I:C)/dsRNA InvivoGen tlrl-picwlv Poly(dA:dT) / LyoVec InvivoGen tlrl-patc Human IL-2 PeproTech 200-02-100 RIPA buffer Cell signaling technology 9806s DAPI Sigma-Aldrich D9542 ADU-S100 MCE HY-12885B Critical commercial assays 2’3’-cGAMP ELISA Kit Cayman 501700 RNA Purification Kit EZBioscience HiScript II QRT SuperMix Vazyme R223-01 ChamQ-SYBR-qPCR-MasterMix Vazyme Q311-02 qPCR Primers (human) Forward (5’>3’) Reverse (5’>3’) ..

    Purification:

    Article Title: A High Immune-Related Index with the Suppression of cGAS-STING Pathway is a Key Determinant to Herceptin Resistance in HER2+ Breast Cancer
    Article Snippet: .. Table S1: Key Resources Antibodies Source Identifier Dilution IRF3 Proteintech 11312-1-AP-100UL 1:1000 HER2 Proteintech 18299-1-AP-50ul 1:1000 Phospho-IRF3 (Ser396) Abcam Ab138449 1:1000 cGAS Proteintech 26416-1-AP-100ul 1:1000 TBK1 Cell signaling technology 38066S 1:1000 AKT1 (B-1) Santa Cruz sc-5298 1:500 Phospho-TBK1 (Ser172) Cell signaling technology 5483S 1:1000 Phospho-AKT (Ser473) Cell signaling technology 4060S 1:1000 GAPDH Proteintech 60004-1-IG-100UL 1:5000 α-tubulin Beyotime AT819 1:5000 Anti-human HLA-A, B, C BioLegend 311406 1:300 CD3 BioLegend 300313 1:500 CD28 BioLegend 302913 1:500 CD8a Proteintech 66868-1-IG 1:300 GZMB Proteintech 13588-1-AP 1:300 Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Invitrogen 11668019 HT-DNA Sigma-Aldrich D6898 2’3’-cGAMP Titan 04800926 Poly(I:C)/dsRNA InvivoGen tlrl-picwlv Poly(dA:dT) / LyoVec InvivoGen tlrl-patc Human IL-2 PeproTech 200-02-100 RIPA buffer Cell signaling technology 9806s DAPI Sigma-Aldrich D9542 ADU-S100 MCE HY-12885B Critical commercial assays 2’3’-cGAMP ELISA Kit Cayman 501700 RNA Purification Kit EZBioscience HiScript II QRT SuperMix Vazyme R223-01 ChamQ-SYBR-qPCR-MasterMix Vazyme Q311-02 qPCR Primers (human) Forward (5’>3’) Reverse (5’>3’) ..

    Article Title: Brain-wide mapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL-17RB.
    Article Snippet: Article Brain-widemapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL17RB

    Real-time Polymerase Chain Reaction:

    Article Title: A High Immune-Related Index with the Suppression of cGAS-STING Pathway is a Key Determinant to Herceptin Resistance in HER2+ Breast Cancer
    Article Snippet: .. Table S1: Key Resources Antibodies Source Identifier Dilution IRF3 Proteintech 11312-1-AP-100UL 1:1000 HER2 Proteintech 18299-1-AP-50ul 1:1000 Phospho-IRF3 (Ser396) Abcam Ab138449 1:1000 cGAS Proteintech 26416-1-AP-100ul 1:1000 TBK1 Cell signaling technology 38066S 1:1000 AKT1 (B-1) Santa Cruz sc-5298 1:500 Phospho-TBK1 (Ser172) Cell signaling technology 5483S 1:1000 Phospho-AKT (Ser473) Cell signaling technology 4060S 1:1000 GAPDH Proteintech 60004-1-IG-100UL 1:5000 α-tubulin Beyotime AT819 1:5000 Anti-human HLA-A, B, C BioLegend 311406 1:300 CD3 BioLegend 300313 1:500 CD28 BioLegend 302913 1:500 CD8a Proteintech 66868-1-IG 1:300 GZMB Proteintech 13588-1-AP 1:300 Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Invitrogen 11668019 HT-DNA Sigma-Aldrich D6898 2’3’-cGAMP Titan 04800926 Poly(I:C)/dsRNA InvivoGen tlrl-picwlv Poly(dA:dT) / LyoVec InvivoGen tlrl-patc Human IL-2 PeproTech 200-02-100 RIPA buffer Cell signaling technology 9806s DAPI Sigma-Aldrich D9542 ADU-S100 MCE HY-12885B Critical commercial assays 2’3’-cGAMP ELISA Kit Cayman 501700 RNA Purification Kit EZBioscience HiScript II QRT SuperMix Vazyme R223-01 ChamQ-SYBR-qPCR-MasterMix Vazyme Q311-02 qPCR Primers (human) Forward (5’>3’) Reverse (5’>3’) ..

    Virus:

    Article Title: Brain-wide mapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL-17RB.
    Article Snippet: Article Brain-widemapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL17RB

    Electron Microscopy:

    Article Title: Brain-wide mapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL-17RB.
    Article Snippet: Article Brain-widemapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL17RB

    Control:

    Article Title: Brain-wide mapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL-17RB.
    Article Snippet: Article Brain-widemapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL17RB

    Blocking Assay:

    Article Title: Brain-wide mapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL-17RB.
    Article Snippet: Article Brain-widemapping of immune receptors uncovers a neuromodulatory role of IL-17E and the receptor IL17RB

    other:

    Article Title: In0testinal stem and progenitor cells exhibit distinct adaptive responses to inflammatory stress in IBD.
    Article Snippet: Antibodies and dyes Company Catalog Dilution S100A9 Proteintech 26992-1-AP 1:200 DAPI Scientific Laboratory D9542-1MG 1:500 OLFM4 Cell Signalling D6Y5A 1:200 β-catenin Santacruz Biotech sc-7963 1:500 858 Table 3.



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